Ability of Integrins to Mediate Fertilization, Intracellular Calcium Release, and Parthenogenetic Development in Bovine Oocytes1
نویسندگان
چکیده
منابع مشابه
Intracellular calcium release at fertilization in the sea urchin egg.
Fertilization or ionophore activation of Lytechinus pictus eggs can be monitored after injection with the Ca-sensitive photoprotein aequorin to estimate calcium release during activation. We estimate the peak calcium transient to reach concentrations of 2.5-4.5 & free calcium 45-60 set after activation and to last 2-3 min, assuming equal Ca2+ release throughout the cytoplasm. Calcium is release...
متن کاملIntracellular calcium in the fertilization and development of mammalian eggs.
1. Mammalian eggs are arrested at metaphase of their second meiotic division when ovulated and remain arrested until fertilized. The sperm delivers into the egg phospholipase C (PLC) zeta, which triggers a series of Ca(2+) spikes lasting several hours. The Ca(2+) spikes provide the necessary and sufficient trigger for all the events of fertilization, including exit from metaphase II arrest and ...
متن کاملIntracellular calcium release and the mechanisms of parthenogenetic activation of the sea urchin egg.
Parthenogenetic activation of Lytechinuspictus eggs can be monitored after inje&ion with the Ca-sensitive photoprotein aequorin to estimate calcium release during activation. Parthenogenetic treatments, including the nonelectrolyte urea, hypertonic sea water, and ionophore A23187, all acted to release Ca2+ from intracellular stores. Ionophore and urea solutions release Ca*’ from the same intrac...
متن کامل1. Assessment of quercetin ability to mediate calcium release in Jurkat lymfoblasts
The present report structure follows the Work Plan proposed for the fourth stage of the project (Act Adițional 1/2014). Supplementary to the objective proposed and fulfilled, we have performed more studies, that are continuing researches carried out in the third stage of the project and completing them. Nearly all the results currently reported have been communicated at national and internation...
متن کاملA comparative study of parthenogenetic activation and in vitro fertilization of in vitro matured bovine oocytes
The objective of this study was to determine the effects of 3 chemical agents used sequentially and electrical stimuli on parthenogenetic activation of in vitro matured bovine oocytes and comparison with a standard IVF protocol for embryo developmental rates. For this purpose, oocytes were matured in tissue culture medium-199 (TCM-199) at 39 °C and 5% of CO2 in humidified air. For IVF, matured ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biology of Reproduction
سال: 2000
ISSN: 0006-3363,1529-7268
DOI: 10.1095/biolreprod62.6.1702